Article Per Year (5 Year)
p-Index From 2019 - 2024
0.562
P-Index
This Author published in this journals
All Journal JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Makara Journal of Science
Winda Nawfetrias, Winda
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Immunology & microbiology

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Makara Journal of Science

 1 
Identification of MADS-box Gene in Oil Palm (Elaeis guineensis Jacq.) Nawfetrias, Winda; Sobir,; Faizal, Irvan
Makara Journal of Science Vol. 20, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The bunch size represented by the fruit number is the main parameter of oil palm (Elaeis guineensis Jacq.) yield. The fruit number, which is determined during the initial phase of development, is related to various factors, including the genetic properties of the trees. Trees that have more pistillate flowers have more fruit. The diversity of MADS-box genes assumed can be used as a marker for trees that have a higher number of pistillate flowers. Therefore, the aims of this research were to isolate and identify the MADS-box genes from flowers of tenera oil palm using PCR techniques. The SQUAMOSA (SQUA) gene and the GLOBOSA (GLO) gene are members of the MADS-box genes family that are responsible for sepal, petal and stamen organ development. The genomic DNA of the staminate flowers of trees that have more staminate flowers (P1) and the genomic DNA of the pistillate flowers of trees that have more pistillate flowers (P2) were isolated using the CTAB+ PVP method. The CTAB+PVP method was more efficient for isolating pistillate flower genomic DNA than staminate flower genomic DNA. The genomic DNA of P1 and P2 was amplified with two primers: BMS and BMG. The BMS primers gave a PCR product size of 1250 bp for the genomic DNA of P1 and P2. Meanwhile, the BMG primers gave a PCR product size of 1250 bp and 1300 bp for P1 and P2, respectively. The PCR products were sequenced and analyzed for homology using the GenBank database. BLAST analysis showed the PCR products have high homology with the SQUA1 gene and the GLO2 gene. Alignment analysis showed that the DNA fragments amplified with the BMS primers of the P1 and P2 sequences have variations in the exons and introns, and the variations were observed only in the introns of the DNA fragments amplified with the BMG primers.
Co-Authors Armelia Tanjung, Armelia Bidara, Irna Surya DWI PANGESTI HANDAYANI, DWI PANGESTI Eka Nurhangga, Eka Henti Rosdayanti, Henti Irvan Faizal Lukita Devy Pinardi, Djatmiko Pinardi, Djatmiko Sobir, Sulastri Sulastri Sutardjo ., Sutardjo Sutardjo Sutardjo